The dataset and analysis scripts provided were essential for article. Article aims to enhance the understanding of factors that influence mobility satisfaction and the use of motorised transport modes by older adults in Czechia within the context of gender equity. We used data from a large questionnaire survey that explored the main trends in the mobility behaviour, needs, and attitudes of the Czech population, focusing on key gender mobility issues. The responses of 2,087 participants aged 65 years and older were analysed using regression models. Our findings confirm significant gender differences in car use. Older women in Czechia use public transport more and drive cars less than their male counterparts. Additionally, they are less satisfied with their overall transport options. However, in the subsequent regression analysis, gender did not significantly affect transport satisfaction. Instead, the major factors influencing transport satisfaction in our research were age, physical disability, place of residence, car accessibility, and income. The gender distribution of the last two mentioned variables showed substantial disparities with a more negative impact on women, which likely resulted in lower overall transport satisfaction.