Infections are a major cause of mortality in newborns and advances in neonatal management have led to considerable improvement in newborn survival. Late onset fungal sepsis is a devasting disease in extreme low birth weight infant, has high morbidity and mortality. It is associated with with pronged hospital stay and increased health care costs.Amphotericin is often started when invasive candidiasis is suspected and it has own side effects. Antifungal susceptibility testing can be useful in deciding correct antifungal therapy. This case report highlights the rising cases of candida nonalbicans in neonatal intensive care units and antifungal susceptibility testing to decide the optimal antifungal therapy.