Data for submission to Wellcome Open Research entitled "ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy". Data_ultraLM.tif is an image stack from the fluorescence microscope mounted on the ultramicrotome. Data_miniLM.tif is an image stack from the fluorescence microscope mounted in the SBF-SEM. Data_miniLM_EM.tif is an image stack from the SBF-SEM while the miniLM was in-situ.

This work was supported by the Francis Crick Institute which receives its core funding from the Wellcome Trust [FC001999]; Cancer Research UK [FC001999]; the UK Medical Research Council [FC001999]; and from the UK Medical Research Council, BBSRC and EPSRC under grant [MR/K01580X/1].