Contaminated food, particularly by food-borne pathogens such as Salmonella and Listeria, representsa significant global public health challenge, leading to severe gastrointestinal illnesses and thousands of fatalitiesannually [1]. Traditional pathogen detection methods are labor-intensive, time-consuming, and requirespecialized equipment and trained personnel. Rapid and accurate pathogen detection on site is vital for ensuringenvironmental and food safety and consequently public health. Electrochemical detection using gold electrodeswith magnetic nanoparticles offers a promising platform for rapid and sensitive pathogen detection which allowsincorporation of pre-concentration step as well. By functionalizing magnetic nanoparticles with specific aptamersor antibodies, this method achieves high specificity for targeted pathogens, without the the necessity of directbiomolecule immobilization onto the electrode. This study presents an innovative approach utilizingnanoparticles functionalized with aptamers to detect Listeria and Salmonella on a low-cost gold leafelectrochemical transducing platform [2]. Electrochemical impedance spectroscopy (EIS) was used to detectvarying bacterial concentrations in both, buffer and complex matrices. The developed sensor demonstratedspecific recognition of Listeria and Salmonella, achieving a detection limit of 10¹ cfu/mL. The results demonstratea clear and linear increase in impedance with rising bacterial concentrations, confirming the sensitivity andeffectiveness of the platform.[1] World Health Organization, Gobal strategy for food safety 2022-2030: towards stronger food safety systems andglobal cooperation, WHO, 2022.[2] I. Podunavac, M. Kukkar, V. Léguillier, F. Rizzotto, Z. Pavlovic, Lj. Janjušević, V. Costache, V. Radonic, J. Vidic.2023, Low-cost goldleaf electrode as a platform for Escherichia coli immunodetection, Talanta 259.doi:10.1016/j.talanta.2023.124557