The purpose of the study was designed to examine the effect of tempo running on speed and agility of university men students. For the purpose of the study, thirty university men students studying bachelor’s degree in Karnataka University, Darwad, Karnataka State, India were selected as subjects. They were divided into two equal groups. each group consisted of the fifteen subjects. Group I underwent tempo running for three days per week for twelve weeks. Group II acted as control who did not undergo any special training programme apart from their regular physical education programme. The following variables namely speed and agility were selected as criterion variables. All the subjects of two groups were tested on selected dependent variables by using leg lift with dynamometer and back lift with dynamometer respectively at prior to and immediately after the training programme. The analysis of covariance was used to analyze the significant difference, if any among the groups. The .05 level of confidence was fixed as the level of significance to test the ‘F’ ratio obtained by the analysis of covariance, which was considered appropriate. The results of the study showed that there was a significant difference between tempo running group and control group on speed and agility. And also it was found that there was a significant improvement on speed and agility due to twelve weeks of tempo running.