This repository contains the minimal datasets required to reproduce the morphometric and molecular analyses presented in the manuscript “Cryptic diversity in Astroblepus (Siluriformes: Astroblepidae): integrative taxonomy reveals evolutionary complexity in the Esmeraldas River basin, Ecuador”. It includes raw geometric morphometric landmark coordinates (dorsal, lateral, and ventral views), specimen voucher metadata, and GenBank accession numbers for all sequences analyzed. All tabular data are provided in open CSV format to ensure accessibility and reproducibility. These files allow full replication of the statistical, morphometric, and phylogenetic analyses described in the study.