Background: Poisoning cases constitute a significant proportion of medical and medico-legal emergencies presenting to tertiary care centres in India. Optimal management requires not only clinical expertise but also sound medico-legal knowledge and appropriate practices. Objectives: To assess the knowledge, attitude, and practice (KAP) among healthcare professionals regarding handling of poisoning cases in tertiary care centres and to identify gaps requiring targeted training. Methods: A cross-sectional questionnaire-based study was conducted among 236 healthcare professionals using a pre-validated, anonymous KAP questionnaire. The tool assessed demographic variables, knowledge of poisoning management and medico-legal aspects, attitude toward protocols and training, and real-world practices. Data were analysed using descriptive statistics. Results: Among 236 respondents, interns and residents constituted the largest groups. While basic awareness regarding poisoning cases was satisfactory, gaps were observed in areas such as standardized management protocols, medico-legal documentation, and consistent practice patterns. A positive attitude toward structured training and protocol-based management was noted across all professional categories. Conclusion: Although healthcare professionals demonstrated reasonable knowledge and positive attitudes toward poisoning management, deficiencies in practical implementation persist. Regular training programs, standardized protocols, and reinforcement of medico-legal responsibilities are recommended