KIC Spheroid Calcium Pipeline — Script 01/03 GUI-based R pipeline for preprocessing, reconstructing, and summarizing calcium transient data from 3D cardiac spheroids generated using the CyteSeer Cytometer (Vala Sciences). What's included GUI selection of input/output paths Recursive scanning of CyteSeerResults folders Loading of CyteSeer CSV exports (cell-level and whole-image tables) Reconstruction of calcium waveform time series from KIC strings Computation of additional upstroke and downstroke kinetic parameters Export of structured cell-level and whole-spheroid summary datasets Inputs CyteSeerResults export folders Primary_DataTable_CStats.csv _Cell Peak Measurements_DataTable.csv _Whole Image Measurements_DataTable_CStats.csv _Whole-Image Peak Measurements_DataTable.csv (All files must be generated using the Cardiac_Two-Channel_TimeSeries_Extended algorithm in CyteSeer) Outputs Reconstructed calcium waveform datasets Derived kinetic parameter tables Cell-level summary tables Whole-spheroid summary statistics Cleaned, standardized outputs ready for downstream analysis Citation Developed by Michele Buono, Talitha Spanjersberg, Nikki Scheen, and Nina van der Wilt. Free for research use and adaptation with citation via Zenodo.