In this study, efforts are made to develop a formulation of transdermal patches with the use of Musa paradisiaca extracts as primary pharmaceutically active components to test its possible antimicrobial activity. The agricultural waste, which is rich in bioactive compounds, served as a natural source of medication. In preparing these transdermal patches, hydroxypropyl methylcellulose (HPMC), as well as Carbopol, served as film-gelling polymers. Propylene glycol, on the other hand, worked as plasticizers along with glycerin, which acted as enhancements. Methanol served as a solvent. The prepared transdermal patches were tested for different physiochemical parameters such as organoleptic test, surface pH, thickness, and folding endurance as a measure of their suitability for transdermal delivery. Antimicrobial potential was taken as the main evaluating criterion, which was tested against specific microorganisms to measure the potency of the Musa paradisiaca extract through a transdermal delivery system. This research attempts to explore the feasibility of using Musa paradisiaca skin transdermal patches as an eco-friendly alternative delivery method of antimicrobial compounds. Further research is recommended to improve the formulation of the delivery system and investigate the release kinetics as a means of establishing the feasibility of using Musa paradisiaca as a pharmaceutical compound.