Copper sulphate (CuSO₄) is widely used in agriculture and industries but poses a significant threat to environmental and animal health due to its potential toxic effects. This study aims to investigate the effects of copper sulphate poisoning on sperm parameters, including sperm count, sperm motility, sperm mortality, and sperm morphology, in Mus musculus (house mouse). The study utilized various doses of copper sulphate administered to male mice, and sperm quality parameters were analyzed post-treatment. Results indicate that copper sulphate adversely affects sperm count, motility, mortality, and morphology, suggesting its potential to induce reproductive toxicity in mammals.