This repository contains the data and code associated with the manuscript: The distribution of fitness effects of new mutations in regulatory regions of the D. melanogaster genome Austin Daigle, Jacob Marsh, Andrew Kay, Parul Johri bioRxiv 2026.03.01.708907; doi: https://doi.org/10.64898/2026.03.01.708907 Repository contents 1. BED files specifying genomic coordinates (nonoverlapping_annotations.zip) for the annotation classes used in inference: - exonic regions - phastCons conserved noncoding regions - low-confidence regulatory regions - high-confidence regulatory regions - putatively neutral intergenic regions 2. The final filtered VCF (vcf_for_dfereg_simulansfilter.noRepeats.vcf.gz) used for empirical analyses of the three study populations, together with the final accession list (sample_lists.csv). 3. All code required to reproduce the simulations (simulations.zip) reported in the manuscript, including the mutation and recombination rate maps, along with the simulation outputs analyzed in the manuscript. --- ContactFor questions about these data and scripts, please contact: Austin DaigleUniversity of North Carolina at Chapel Hilladaigle@unc.edu