This poster presents the establishment of the Centre of Preclinical Toxicology and Biochemistry (PreToxBi@) at the Mario Negri Institute to address the growing need for standardized and reproducible nanomedicine safety assessment. As nanoformulations increasingly enhance drug delivery and therapeutic efficacy, concerns regarding their safety and long-term biological effects remain insufficiently addressed. The Centre integrates toxicological and biochemical expertise to evaluate nanomaterial interactions at the molecular level, identify adverse effects, and investigate mechanisms underlying toxicity and chronic disease development. By shifting from risk perception to quantitative measurement, PreToxBi@ aims to provide a structured, translational platform supporting safer development of nanodrugs.