Dengue fever is an arboviral disease transmitted by mosquitos. There are currently no viable vaccinations for this major public health issue in tropical and subtropical regions. Every year, around 400 million people become infected with the Dengue virus, with a fatality rate of about 40% of patients with severe dengue infection. The Dengue virus belongs to Flaviviridae family. Research on this Dengue virus infection cycle identified possible host targets for transmission and replication. In this review, we discuss the structural and non-structural proteins of Dengue virus and their activities.