Data and analysis scripts for Borrero et al.: Sexual dimorphism in pollen foraging and sensory traits in Heliconius butterflies # Reference Information ## Provenance for this README - File name: README_himera_sensory_ecology.md - Authors: José Borrero - Other contributors: David F. Rivas-Sánchez, , Daniel Shane Wright, Stephen H. Montgomery, Alexander Keller, Richard M. Merrill - Date created: 2025-12-01 - Date modified: 2025-12-01 ## Dataset Version and Release History - **Current Version** - Number: 1.0.0 - Date: 2025-12-01 - Persistent identifier: DOI: 10.5281/zenodo.17777739 - Summary of changes: Initial public release of *H. himera* sensory ecology and brain dimorphism datasets; includes brain morphology, eye morphology, reflectance-age calibration, pollen metabarcoding, and microhabitat recapture datasets. - **Previous Versions** - None (this is the first release) - Embargo Provenance: n/a - Scope of embargo: n/a - Embargo period: n/a --- # Sexual dimorphism in pollen foraging and sensory traits in Heliconius butterflies### Data & Analysis Repository This repository contains datasets and analysis scripts used across multiple components of the *Heliconius himera* sensory ecology project, including brain morphology, eye morphology, wing reflectance, pollen metabarcoding, and recapture microhabitat analyses. Files are grouped below by theme and briefly described. --- # Data and File Overview ## 1. Brain Morphology & Sexual Dimorphism ### **Data**- **Cyr_him_combined_brain_volumes.csv** Combined neuropil volume dataset for *H. cydno* and *H. himera* with log-transformed measurements and metadata. - **Hhimera_eyemorph.csv** Eye morphology dataset (ommatidia counts, eye area) for *H. himera*. - **TableS1_Hhim-brain-dimorphism.xlsx** Curated neuropil volume summary table for sexual dimorphism analysis. - **himera_lrt_table.csv** Likelihood-ratio test results for neuropil linear mixed models. - **smatr_table_himera.csv** Table containing slope, intercept, and elevation shift outputs from SMATR. ### **Scripts**- **Brains_sex_him.r** Main statistical pipeline for modelling sexual dimorphism in brain structure. - **smatr_eyes_himera.R** SMATR major axis analysis of eye scaling relationships. --- ## 2. Reflectance Spectrometry & Age Prediction ### **Data**- **reflectance_age.csv** Area-under-curve reflectance measures used to train age prediction models. - **Vilcabamba_samples_age.csv** Known-age dataset for wild *H. himera* from Vilcabamba used to validate reflectance–age mapping. ### **Scripts**- **reflectance_age_modeling_full.R** End-to-end reflectance-based age modelling script (model fitting, validation, diagnostics). --- ## 3. Pollen Metabarcoding & Foraging Ecology ### **Data**- **asv_vilcabamba_table.merge.txt** Merged ITS2 amplicon sequence variant (ASV) table from proboscis and gut samples. - **samples_metadata_vilcabamba.csv** Metadata for sequenced samples (sex, body part, date, barcode, etc.). ### **Scripts** - **metabarcoding_tools_0-1a.R** Helper functions for data cleaning, ASV processing, and taxonomic lookup.- **R_ITS2_vilcabamba.R** ITS2 metabarcoding pipeline including filtering, taxonomic assignment, and diversity analyses. --- ## 4. Microhabitat & Recapture Data ### **Data**- **recaptures_logger_1h.csv** Temperature and relative humidity recorded approximately one hour after recapture events. - **Vilcambamba_recapture_data.csv** Spatial and environmental capture–recapture dataset (coordinates, canopy cover, flight height, temperature, humidity). ### **Scripts** - **Pollen_loads.R** Quantification of pollen loads, taxonomic richness, and Shannon diversity. # Reproducibility Notes - Analyses were performed using **R v4.2–4.4**. - Required packages include: `tidyverse`, `lme4`, `smatr`, `vegan`, `phyloseq`, `ggplot2`, and others listed within individual scripts. - Scripts are modular and will run when corresponding datasets are placed in the working environment. - Raw sequencing FASTQ files are **not** included in this dataset release. - All coordinate data have been processed to remove sensitive location information where needed. --- # Dataset Metadata ## Dataset Title**Sexual dimorphism in pollen foraging and sensory traits in Heliconius butterflies** *(Formal publication title will be updated upon manuscript acceptance.)* ## Persistent IdentifierTo be assigned (10.5281/zenodo.17777739). ## Dataset Contributors - José Borrero (Creator)- David F. Rivas-Sánchez- Daniel Shane Wright - Stephen H. Montgomery - Alexander Keller- Richard M. Merrill ## Date of Issue2025-12-01 ## PublisherTo be updated depending on repository (Dryad, Zenodo, institutional repository). ## License- **CC0 1.0 Universal (CC0 1.0)** - Specification: https://creativecommons.org/publicdomain/zero/1.0/ - The authors respectfully request to be contacted by researchers interested in re-use of these data to discuss potential collaboration. --- # Contact Information ### Primary Contact- **Name:** José Borrero - **Affiliation:** Department of Evolutionary Biology, LMU Munich - **ORCID:** https://orcid.org/0000-0003-0164-496X - **Email:** jose.borrero@lmu.de ### Alternate Contact (PI)- **Name:** Richard M. Merrill - **Affiliation:** Department of Evolutionary Biology, LMU Munich - **ORCID:** https://orcid.org/0000-0003-4527-9298 - **Email:** merrill@bio.lmu.de - **Address:** LMU Biozentrum Martinsried, Großhaderner Straße 2, 82152 Martinsried, Germany --- # Additional Information ## FundingThis research was funded by a European Research Council (ERC) Starter Grant (851040) to R.M.M. ## Dates and Locations of Data Collection- Field data were collected from 2023. - Geographic location: Vilcabamba, Ecuador (Andean dry forest habitat). - Laboratory analyses were performed at LMU Munich. --- # Setup - Recommended software: RStudio (2023 or later), R v4.2–4.4 - Scripts are written in base R and tidyverse conventions. --- # Table of Contents - README.md - Brains_sex_him.r - smatr_eyes_himera.R - reflectance_age_modeling_full.R - R_ITS2_vilcabamba.R - metabarcoding_tools_0-1a.R - Pollen_loads.R - All associated datasets (.csv, .txt, .xlsx) --- **END OF README**