GSL + Ceramide Transition Generator v1.0.4 Bug fixes and improvements release. What's New implemented carboxylate anion [RCOO]- logic for negative mode updated GUI-Backend Synchronization Fixed bug where the mass of water was incorrectly added to negative mode headgroup loss fragments Corrected the m/z calculation for isotope labelling of doubly charged negative fragment ions Fixed the definition for a nLc10 Y-ion transition Downloads Windows Tested (Windows 10/11 Enterprise) GSL-Generator-v1.0.4-Windows.zip Linux Tested (Tuxedo OS/Ubuntu 24.04.3 LTS) GSL-Generator-v1.0.4-Linux.zip macOS (Intel) Untested GSL-Generator-v1.0.4-macOS-Intel.zip - For MacBook Pro 2020 and earlier macOS (Apple Silicon) Untested GSL-Generator-v1.0.4-macOS-AppleSilicon.zip - For M1/M2/M3 Macs (2020+) Installation Instructions Windows Download and extract GSL-Generator-v1.0.4-Windows.zip Run gui_gslgen.exe Note: The Windows executable includes all required runtime libraries. Linux Download and extract GSL-Generator-v1.0.4-Linux.zip Make executable: chmod +x gui_gslgen.bin Run: ./gui_gslgen.bin macOS (Untested) Download the appropriate version for your Mac Intel Macs (2020 and earlier): Use macOS-Intel version Apple Silicon (M1/M2/M3): Use macOS-AppleSilicon version Extract the zip file Open Terminal and run: cd ~/Downloads chmod +x gui_gslgen.bin xattr -d com.apple.quarantine gui_gslgen.bin ./gui_gslgen.bin Note: macOS builds are automatically compiled but haven't been tested on actual hardware. Please report any issues! Features Generate transitions for GSLs (GM1, GM2, GM3, GD1a, GD1b, GT3, GQ1, etc.) Support for Ceramides and deoxyceramides Configurable LCB chains and fatty acids Isotope labeling support (M2DN15, M3D) Multiple adduct types and charge states Positive and negative ion modes System Requirements No Python installation required - standalone executables Windows 10/11 (64-bit) or Linux (64-bit)