The present study focused on the formulation and evaluation of a colon-targeted mucoadhesive herbal tablet containing methanolic extract of Duranta erecta for anthelmintic therapy. The extract demonstrated marked dose-dependent anthelmintic activity against Eisenia fetida, with higher concentrations producing rapid paralysis and mortality, confirming the presence of bioactive phytoconstituents. Safety evaluation using the brine shrimp lethality assay revealed an LC₅₀ value of 140.9 mg/mL, and the formulation dose (10mg) was selected well below this level to ensure safety. Heckel plot analysis indicated optimal plastic deformation and compressibility at 4% PVP K-30, which was selected as the optimum binder concentration. FTIR studies confirmed compatibility between the extract and excipients. The validated UV spectrophotometric method showed excellent linearity (R² = 0.999), precision, and sensitivity. Precompression parameters confirmed good flow and compressibility of granules, while post-compression evaluation demonstrated acceptable hardness, friability, and uniformity. Increased concentrations of Carbopol 934P and sodium alginate significantly enhanced mucoadhesive strength and mucosal retention. The optimized formulation exhibited negligible drug release at gastric and intestinal pH, followed by sustained release at colonic pH. Notably, it produced significantly faster paralysis (⁓15min) and death (53min) times compared to Albendazole, (72 min & 83 min) confirming its potential as an effective colon-targeted anthelmintic formulation