Nowadays, sustainability plays a leading role in the textile and fashion industry due to the huge amount of consumption of energy, hazardous chemicals, and water being used in the above industries. Many textile and fashion industries are focusing more on eco-friendly products which help to meet the environmental and social aspects. From the last few decades, denim has become the most preferable product in the fashion industry adopted by all gender and age groups but at the same time, denim manufacturing has caused a huge environmental hazardous impact in terms of water and carbon footprints. Moreover, cotton is the main constituent of denim products which is also responsible for environmental hazardous impact as cotton production uses 25 % of insecticides and 12% pesticides which causes a concern for human health. In this study, an attempt has been made to reduce environmental hazardous impact by developing sustainable textile material made from bio-based renewable resources in the form of bamboo fibres. In this research study, Denim fabric samples are developed using ring yarn of 100% bamboo fibre with 2/20 Ne in a weft way and 100% cotton fibre with 2/20 Ne in the warp way on handloom frame with 2/1 and 3/1 twill weave. It is observed that lesser crease recovery, lower flexural rigidity, higher elongation % and tear strength using bamboo than cotton yarn. It is also found that better flexibility, higher air permeability, higher tensile and tearing strength in 3/1 twill weave as compared to 2/1 twill woven fabrics.