DescriptionThis record contains filtered single cell RNA sequencing data from Dictyostelium discoideum used in the manuscript: “Early cyclic adenosine monophosphate signaling orchestrates single cell synchronicity throughout Dictyostelium development” by Katoh Kurasawa, Trnovec et al. FilesThree AnnData objects in .h5ad format (compatible with Python and Scanpy) are provided: AX4.h5ad acaA.h5ad acaA_pkaC.h5ad Each file includes the filtered expression matrix and associated cell and gene metadata used for downstream analyses in the manuscript, including clustering, annotation, trajectory analyses, and synchronicity quantification. Relationship to raw dataRaw sequencing data and primary submission files are available through the Gene Expression Omnibus repository under accession [GSE305468]. Reproducible analysis codeThe analysis pipeline corresponding to this dataset is archived as Zenodo software record https://doi.org/10.5281/zenodo.18481808 and is also available on GitHub at https://github.com/lenatr99/scRNA_dicty. NotesThese .h5ad files represent processed and filtered data intended for analysis and figure reproduction. For full raw data and submission metadata, please use the Gene Expression Omnibus accession listed above.