Report about green infrastructure (GI) in Czech regions: Jihočeský, Královéhradecký and Vysočina. It describes the present and past distribution of GI, changes in connectivity, distribution of main groups of ecosystem services, relationship between GI and general and special nature protection areas, causes of lack of GI. In the localities with lack of GI, it analyses in detail land ownership (municipalities and state), state of land consolidation projects, occurrence of protection areas and occurrence of GI in the past. It groups localities with lack of GI into different groups based on aforementioned criteria, which form prioritization types for increasing the amount of GI in these localities.