The US NEXRAD radar network has monitored the aerosphere over the US and its territories since the 1990s and archived over 290 million files that contain a wealth of information about the movements of birds, bats, and insects. We extracted measurements of biological activity from all files in the NEXRAD archive. This data record contains vertical profiles of aerial biomass from 2000 through 2004. Vertical profiles for other years and time series data products can be found in related works. Please visit https://github.com/darkecology/darkeco-dataset to access the full dataset, including download links, documentation, and examples.