Part I: 10.5281/zenodo.18347876 Part II: 10.5281/zenodo.18360348 Part III: 10.5281/zenodo.18362153 This dataset is one out of three zenodo entries encompassing a PIE-FLIM dataset offull-length human FoxP1, point-mutation and truncation variants acquired in live HEK293T cells. This dataset contains(i) the Python script used to analyse the data(ii) the raw data (Picoquant .ptu format)(iii) binary masks used to the select the region of interest All other data (e.g. fluorescence intensity decays), sub-masks and values can be created from the raw data and the masks using the provided scripts. A detailed description of how to install the required packages for the scripts and how to run themwas published in a similar project: 10.5281/zenodo.17869433 The 3 zenodo entries contain the following datasets: Part I: khm_20231117_FoxP: Full-length, wildtype FoxP1khm_20231201_FoxP: ZIP-FKH truncation variant wildtype of FoxP1 or including R514H mutationkhm_20231208_FoxP: ZIP-FKH truncation variant wildtype of FoxP1 or including the delta D358 deletionkhm_20240308_FoxP: Full-length, wildtype FoxP1 with R514H mutation Part II: khm_20231213_FoxP: ZIP-FKH truncation variant of FoxP1 including the delta D358 deletion or the R514H mutationkhm_20231124_FoxP: Full-length, wildtype FoxP1 and including the delta D358 deletionkhm_20231129_FoxP: Full-length, wildtype FoxP1 including the delta D358 deletion or the R514H mutationkhm_20231220_FoxP: FKH truncation variant of FoxP1 wildtype or including R514H mutation Part III: khm_20231215_FoxP: FKH truncation variant of FoxP1 wildtype or including R514H mutation