Abstract Language barriers substantially hinder healthcare outcomes, especially among vulnerable groups such as pregnant women. This study evaluated the perceived impacts of language barriers on quality healthcare delivery for pregnant women attending selected primary health centers in Lagos State, Nigeria. Specific objectives included assessing participants' knowledge of language barriers, their perceived effects on healthcare quality, and the underlying influencing factors. Using an accidental sampling method, 132 pregnant women were recruited, with 130 questionnaires validated for analysis. Data were gathered via a self-designed questionnaire and analyzed descriptively using frequency distributions, percentages, bar charts, SPSS version 23, and Microsoft Excel 2010. Results indicated a high overall knowledge level of language barriers among participants. Key perceived effects on healthcare quality encompassed delayed interventions (93.8%), medication misuse (58.5%), challenges in decision-making (52.3%), strained providerpatient relationships (48.5%), and avoidable costs (20.0%). Influencing factors included cultural disparities (90.8%), low educational attainment (80.0%), lack of interpreter services (57.7%), and limited availability of educational materials (5.4%). Statistical analysis revealed significant associations between education level and knowledge of language barriers (p<0.05p<0.05), as well as between knowledge and perceived effects (p<0.05p<0.05). These findings underscore the necessity for targeted communication strategies, such as interpreter services and culturally sensitive care, to support linguistically diverse pregnant women.