In the present study the neurotoxicity of polio vaccine was checked by orally administering the polio vaccine to day seven 7 mice neonates. Different doses (in drops form) were administered to the mice neonates. The doses were low, moderate and high as per number of drops such as 1, 2 and 4 respectively. Postnatal day 7 (PND-7) mice pups were divided into four groups including i. Control (C), ii. Low Dose (LD), iii. Moderate Dose (MD), iv. High Dose (HD). Due to its importance in research this model is compatible with 3rd trimester of pregnancy in the human beings.All the pups were sacrificed after 4 hours of the polio vaccine administration. The brain was collected very carefully. The brains from all the animals were subjected to Western blot technique. The results indicated there was no significant difference among the expression of proteins involved in the neuro inflammation and neurodegeneration in the first three groups like C, LD and MD. In contrast to this the high dose induced significant neurotoxicity in the mice brain and the expression levels of neuro inflammatory and neurodegeneration proteins were significantly high as compared to the control and other treated groups. These proteins include phosphorylated JNK (C-Jun Terminal Kinase), NF-kB (Nuclear factor kappa B) and PARP-1 (Poly (ADP-ribose) polymerase 1). In summary this study shows that among the four groups LD and MD are nontoxic while the HD is neurotoxic to the immature mice brain, hence it is a lethal dose in case compared to the human beings. Keywords: Oral Polio Vaccine, Neuroinflammation, Mice Neonates, p-JNK, NF-?B, PARP-1, Neurotoxicity