This dataset contains anonymized responses from a cross-sectional survey designed to assess Media and Information Literacy (MIL) within a university community. The study targets three groups: students, academic staff (teaching and research personnel), and administrative and technical staff. The questionnaire instruments are aligned with the UNESCO MIL framework and collect information on participants’ self-reported competencies and practices related to accessing, evaluating, creating, and ethically using information in contemporary digital environments. The dataset includes item-level responses to multiple sections covering core MIL dimensions (e.g., critical evaluation of information, knowledge of media systems, ethical and responsible information use, and practical/digital skills), along with sociodemographic and contextual variables (e.g., role within the university and other non-identifying characteristics). Data were collected through structured online questionnaires and processed to minimize risks of re-identification, including the removal of direct identifiers and the aggregation or recoding of potentially sensitive variables where necessary. The dataset can be used for descriptive analyses, group comparisons across university populations, scale construction and validation, and research on determinants of MIL levels and patterns of information behavior. It is suitable for replication studies, methodological work on MIL measurement, and comparative research across institutions or countries, provided equivalent instruments are used.