Subfamily classification within the Lampyridae, and generic categories in the Luciolinae are reviewed, as are the difficulties that often exist with accurate species determinations because of inadequate definition of the genus Luciola LaPorte. A key to genera of the Luciolinae reflects current phylogenetic trends and includes the first morphological details of Luciola (Photuroluciola) (Pic). An expanded generic definition of Pygoluciola Wittmer includes an extension of its geographic range. Pygoluciola qingyu sp. nov. is described including morphology of males, females (bursa structure) and larvae, and the first record of synchronous flashing from mainland China. Behaviour including predacious activity by larvae on large mandibulate ants, in an apparently hazardous frontal attack, the semiaquatic mode of life and luminous activity including synchronous flashing is described and discussed. A lectotype male of Luciola cerata Olivier is designated and described.