Abstract This study assessed the knowledge, acceptability, and factors influencing the uptake of cervical cancer screening among female undergraduates of Bamidele Olumilua University of Education, Science and Technology, Ikere Ekiti. Findings revealed that most respondents possessed a moderate to good understanding of cervical cancer and its preventive measures, with digital media emerging as the primary source of information, supplemented by health workers and peers. Awareness of screening methods, preventive strategies, and risk factors was generally high, yet gaps in comprehensive knowledge were evident. The study also demonstrated a positive disposition toward cervical cancer screening, as a majority expressed willingness to undergo screening, advise family members, and recognize the importance of preventive testing even in the absence of symptoms. Despite this, a minority of respondents exhibited apprehension, reflecting the impact of fear, sociocultural beliefs, and misconceptions. Moreover, the uptake of screening was influenced by a multifaceted interplay of economic constraints, knowledge deficits, accessibility of services, personal beliefs, and health system-related factors. The findings highlight the need for targeted health education, youth-friendly service delivery, and strategies to enhance accessibility and affordability, aiming to strengthen preventive behaviors and early detection practices among young female undergraduates. Keywords: Cervical cancer, Screening, Knowledge, Acceptability, Female undergraduates, Health education,