The scaling process of obtaining an enzyme preparation of exopolygalacturonase (Exo-PgS) from the culture of Lentinus edodes (Berk.) Singer 480 has been studied. The maximum values of Exo-PgS were observed on the 10th day of cultivation, both on small and large volumes of the nutrient medium, with a significant maximum on a medium volume of 1 000 ml. The enzyme preparation Exo-PgS from the culture of the fungus L. edodes 480 is thermally unstable, but short-term heating (15 minutes) of the enzyme preparation solution causes some stabilization of the protein molecules of the Exo-PgS enzymes. The studied enzyme preparation of exopolygalacturonase action includes acidic and neutral exopectinases of directed action, which have an optimum pH of activity of 4.4–4.8 and 6.8–7.2.