The purpose of the study was to find out whether there was any significant difference in stress among the football players at various playing positions. In this study, the researcher has selected thirty-six football players from Tiruchirappalli, Tamil Nadu. The subjects age ranged from 18 to 25 years. Among the thirty-six players 12 were defenders, 12 were midfielders and 12 were attackers. To assess the level of stress, Everly and Girdano’s questionnaire was used. The data collected were statistically analysed. The test the significant differences between the interuniversity teams Analysis of variance F-ratio was computed. The level of significance was fixed at 0.05 level of confidence for the degree of freedom 2 and 33. It was observed that there were insignificant differences in stress among football players at various playing positions.