Table 2. Genes used in this study with PCR primers, primer DNA sequence, optimal annealing temperature and corresponding references. LocusDefinitionPrimersPrimer DNA sequence (5'-3')Optimal annealing temp (°C)References of primers usedITSinternal transcribed spacer of ribosomal RNAITS1TCCGTAGGTGAACCTGCGG51White et al. 1990ITS4TCCTCCGCTTTTGATATGCLSUlarge subunit of ribosomal RNALRORACCCGCTGAACTTAAGC55Vilgalys and Hester 1990LR7TACTACCACCAAGATCTactactinACT-512FATGTGCAAGGCCGGTTTCGC61Carbone and Kohn 1999ACT-783RTACGAGTCCTTCTGGCCCATrpb2RNA polymerase II second largest subunitRPB2-5FGA(T/C)GA(T/C)(A/C)G(A/T)GATCA(T/C)TT(T/C)GG52Liu et al. 1999RPB2-7cRCCCAT(A/G)GCTTG(T/C)TT(A/G)CCCATtef-1αtranslation elongation factor 1-alphaEF1-668FCGGTCACTTGATCTACAAGTGC55Alves et al. 2008EF1-1251RCCTCGAACTCACCAGTACCGtub2beta-tubulinBt2aGGTAACCAAATCGGTGCTGCTTTG55Glass and Donaldson 1995Bt2bACCCTCAGTGTAGTGACCCTTGGC

Published as part of Zhu, Haiyan, Pan, Meng, Bezerra, Jadson D. P., Tian, Chengming & Fan, Xinlei, 2020, Discovery of Cytospora species associated with canker disease of tree hosts from Mount Dongling of China, pp. 97 in MycoKeys 62 on page 97, DOI: 10.3897/mycokeys.62.47854