Abstract Dentine hypersensitivity (DH) is a problem which affects 8% to 57% of the adult dentate population and peaks during the third and fourth decades of life. No single professionally applied desensitizing agent is superior to others in managing dentine hypersensitivity. The management of DH is being investigated with the remineralisation potential of agents in dentinal tubules. This study compared the efficacy of Pro-argin and Calcium fluorophosphosilicate on dentinal hypersensitivity post non-surgical periodontal therapy. A randomised, triple-blinded trial will be conducted with 34 participants clinically diagnosed with DH and equally randomized into two groups with parallel treatment assignment of Pro-argin and Calcium fluoro phosphosilicate based over the counter dentifrices and tested for DH with air blast, mechanical, and water jet stimuli on SCHIFF cold air sensitivity scale (SCASS) and visual analogue scale (VAS) at interim efficacy intervals of 1, 2, 4, and 6-weeks subsequently. Both Pro-Argin and Calcium Fluorophosphosilicate dentifrices effectively reduced dentinal hypersensitivity following non-surgical periodontal therapy. However, the CFPS-based dentifrice demonstrated slightly superior long-term clinical outcomes.