Draft deliverable pending EC approval In recent years, there has been a growing emphasis on developing more sustainable and environmentally friendly methods for producing biopolymers like chitosan (a biopolymer derived from chitin). Traditional chitosan production methods typically involve harsh chemicals and high-energy processes, which can be detrimental to both the environment and human health. Two types of chitosan with different degrees of deacetylation (DD) were produced from an enzymatically produced chitin-rich fraction (500 g) using mild conditions. By adjusting the deacetylation time, chitosan with a low DD (59%) and a higher DD (≥75%) were prepared, with a recovery of 38-45% of 500 g starting chitin-rich fraction. The quality and properties of the low DD chitosan were confirmed by nuclear magnetic resonance (NMR), while characterization of the higher DD chitosan is yet to be conducted. Both types of chitosan were soluble in water. Despite one mild chemical step still being involved, this method offers a significantly more environmentally friendly approach to chitosan production compared to traditional methods.