ABSTRACT Current Mars life-support proposals assume biological closure without addressing the critical engineering challenge: absolute containment in a confined, high-consequence environment. This report presents the first Closed Artificial Ecosystem (CAE) architecture with integrated biosafety protocols, treating organism escape and system failure as design constraints rather than afterthoughts. Key Innovation: A 4-level containment hierarchy ranging from open cultivation chambers to triple-sealed negative-pressure bioreactors, enabling safe integration of high-risk/high-value organisms previously excluded from space agriculture proposals. Critical selections include Hermetia illucens (Black Soldier Fly) larvae for human waste bioconversion (eliminating 36 tonnes/year disposal problem), Tetragonisca angustula (stingless Jataí bees) for pollination in confined spaces, and tilapia-based aquaponics closing the nitrogen cycle without synthetic fertilizers. Quantitative Results: A 100-person pioneer colony achieves >95% nutritional self-sufficiency with 15,000-20,000 m² of intensive cultivation, requiring 1-2 MW continuous power input while maintaining >98% water recycling and >95% nutrient closure. The system includes explicit failure modes and mitigation strategies: insect escape probability (Low, catastrophic impact), genetic degeneration (High, medium impact), and psychological diet fatigue (High, medium impact) with corresponding engineering controls. Implementation Timeline: Conservative 10-year phased deployment from full Earth dependence (Years 0-2) through gradual biological integration (Years 2-5), expansion (Years 5-10), to operational self-sufficiency (Year 10+). Unlike optimistic 2-3 year projections in existing literature, this timeline reflects biological system stabilization requirements validated by terrestrial closed-environment projects (BIOS-3, Biosphere 2). Critical Exclusions: Comprehensive banned-organism list eliminates infesting insects (ants, termites, cockroaches), disease vectors, and any species requiring routine pesticide intervention—a selection criterion absent from competing proposals yet essential for long-term habitat integrity. This work provides implementable technical specifications, mass-energy flow diagrams, risk matrices with quantified probabilities, and organism-specific containment protocols. Complete system architecture addresses the engineering reality that Mars colonization depends not on growing food, but on growing food safely, reliably, and indefinitely in the solar system's most unforgiving environment. Keywords: Closed Artificial Ecosystem, Biosafety Containment, Black Soldier Fly Bioprocessing, Space Agriculture, Martian Life Support, Risk Mitigation, Hermetia illucens, Aquaponics, ISRU Biology