ABSTRAC Urolithiasis commonly referred to as kidney stones occurs due to the intricate connection of dietary practices, environmental factors, genetics and urinary tract anatomy which varies substantially with prevalence rising globally as a consequence of lifestyle changes. Ayurvedic medicinal herbs offer a natural and holistic approach in managing urolithiasis, minimizing recurrence and side effects, compared to allopathic treatments, which often carry risks like gastrointestinal disturbances and renal toxicity. In this regard a unique blend of three medicinal herbs- Aerva lanata, Tribulus terrestris, and Eugenia jambolana are combined to improve the therapeutic effectiveness which showed amalgamation revealing phytoconstituents that had stone dissolving properties confirmed through FTIR studies.This herbal blend in liquid form is a novel approach to treat calculi that occur in the urinary tract with genuine attempt being made to create a novel liquid formulation which achieved a synergistic effect that showed the removal of calculi from the urinary system yet retaining safety. The synergistic effect of liquid formulation was studied by taking male albino rats and administrating 0.75 % v/v of Ethylene glycol mixed with purified water to induce urolithiasis. The administration of the liquid formulation was given from day 15-28 and the analysis of biochemical parameters like oxalate, calcium and phosphate from urine samples and calcium, uric acid and creatinine from serum were analyzed. The study revealed the anti-urolithiatic activity of the liquid formulation through inhibition of biochemical parameters which supports treatment of urolithiasis. Keywords: Urolithiasis, Aerva lanata, Tribulus terrestris, Eugenia jambolana, FTIR spectrum, Phytoconstituents.