This study examines the influence of educational attainment and cultural beliefs on the practice of exclusive breastfeeding (EBF) among working nursing mothers attending immunization clinics in the Akwa Ibom North-West Senatorial District. A descriptive survey design was employed, and 200 working nursing mothers were selected using a multi-stage sampling procedure from general hospitals within the district. Data were collected through a validated, researcher-developed questionnaire titled Knowledge, Attitude, and Practice of Exclusive Breastfeeding Questionnaire (KAPEBQ), with reliability coefficients ranging from 0.71 to 0.88. Data analysis utilized descriptive statistics and inferential tests at a 0.05 significance level. The findings indicated that educational attainment significantly influenced mothers' likelihood of practicing EBF, with higher-educated mothers showing better adherence to recommended practices. Cultural beliefs also played a significant role in breastfeeding decisions, particularly those related to colostrum disposal, pre-lacteal feeding, and family-driven feeding norms. The study concludes that both educational level and cultural beliefs are critical factors shaping EBF behavior among working mothers. It recommends strengthening community-based health education, offering culturally sensitive breastfeeding counseling, and implementing workplace support systems to improve EBF uptake in the district.