This dataset contains all raw fluorescence and processed oxygen consumption data underlying the figures presented in the manuscript: “Liquid-Suspendable Metabolic Sensors Allow Real-Time Oxygen Consumption Monitoring in Caco-2 and Colon Organoid-Based Gut-on-a-Chip Models.” The data were generated using liquid-suspendable metabolic oxygen sensors to monitor time-resolved oxygen consumption in a commercially available microfluidic gut-on-a-chip platform. Measurements were performed in empty chips, Caco-2 epithelial tubules, and adult stem-cell–derived colon organoid models under basal conditions and during mitochondrial stress testing using oligomycin, FCCP, and antimycin A/rotenone. All datasets required to reproduce Figures 1–4 of the manuscript are provided as Microsoft Excel (.xlsx) files, including: Raw fluorescence intensity traces Fitted model outputs Derived oxygen consumption rates (OCR) Mito stress test profiles Transepithelial electrical resistance (TEER) data Spare respiratory capacity (SRC) calculations The raw measurements are organized per experiment and per chip unit, with time-stamped fluorescence values and corresponding experimental conditions. Methods Summary Fluorescence measurements were acquired at 10-second intervals during static (no-flow) incubation phases in microliter-scale microfluidic channels, followed by re-oxygenation via controlled plate tilting. Oxygen consumption rates were calculated from the first derivative of fitted fluorescence curves. Basal and FCCP phases were fitted using five-parameter logistic (5PL) models; oligomycin and antimycin A/rotenone phases were fitted using linear models.