Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a group of gram-positive bacteria that are genetically distinct from other strains of Staphylococcus aureus. MRSA is responsible for several difficult-to-treat infections in humans. Objectives: To assess the knowledge regarding Methicillin Resistant Staphylococcus aureus (MRSA) among nurses of selected hospital of Kathmandu. Methodology: A descriptive cross-sectional design was used. Fourty one nurses from Stupa Community Hospital were selected using enumerative sampling. A self-administered structured questionnaire was used to assess the knowledge level. Data analysis was conducted using SPSS v25, employing descriptive and inferential statistics. Results: The majority of respondents (53.7%) demonstrated a high level of knowledge, while 19.5% had a moderate level, and 26.8% showed a low level of knowledge. This indicates that over half of the participants were well-informed about MRSA, though a notable portion still had limited understanding, highlighting the need for continued education and awareness efforts. Conclusion: More than half of the respondents had adequate knowledge regarding MRSA, though a notable portion still had limited understanding, highlighting the need for continued education and awareness efforts