This is a full dataset associated with a paper preliminarily called "Subjective and psychophysiological response to pictures of ancestral and modern threats: not all evolutionary threats are alike". The paper is authored by Iveta Štolhoferová, Tereza Hladíková, Markéta Janovcová, Šárka Peterková, Daniel Frynta, Eva Landová. All authors affiliate to Department of Zoology, Faculty of Science, Charles University, Viničná 7, 128 43 Prague 2, the Czech Republic. Author identifiers: IŠ: https://orcid.org/0000-0002-1019-0499 TH: https://orcid.org/0009-0004-4801-5997MJ: https://orcid.org/0000-0001-7647-8148ŠP: https://orcid.org/0000-0001-9073-1613DF: https://orcid.org/0000-0002-1375-7972EL: https://orcid.org/0000-0001-8365-8710 Uploaded files: READ_ME.txt: Description of uploaded files. Raw_GSR_Data.zip: Raw data, 119 individual CVS files, each containing full GSR (galvanic skin resistance) measurement session from one participant of the study. Currated datset.xlsx: Two datasheets of currated data used for subsequent statistical analyses. Figure1.png: Design of the experiment and examples of experimental and control stimuli.