Nanocellulose has gained increasing attention in biomedicine for its exceptional biocompatibility, mechanical strength, and potential in wound healing and drug delivery systems. In this study, in this study, we aimed to extract nanocellulose from Arachis Hypogaea plant as a sustainable and low-cost raw material for the production of nano-based biomaterials. experiments were conducted with n=5 biological replicates (human plasma samples from different donors) with triplicate technical replicates per condition. Various analytical techniques were employed, including scanning electron microscopy (SEM) for surface and nanoscale characterization of cellulose nanoparticles The nanocellulose demonstrated significant concentration-dependent effects across all tested parameters (n=5 biological replicates). Antioxidant activity increased from 83.2% ± 2.1% at 15 mg/mL to 91.4% ± 1.8% at 20 mg/mL (p=0.0037), surpassing vitamin C control (84.0% ± 1.5%, p=0.0082). Coagulation studies revealed dose-responsive anticoagulant activity, with PT extending from 15.0 ± 0.8 s (control) to 36.1 ± 1.2 s at 250 mg/mL (p<0.0001) and PTT showing greater sensitivity (39.2 ± 1.1 s to 99.3 ± 3.5 s at 250 mg/mL, p<0.0001), indicating potent intrinsic pathway inhibition. All comparisons were significant by one-way ANOVA with Tukey's post-hoc test (p<0.05). This compound holds promise for the treatment of blood clotting disorders.