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ZENODO
Article . 2015
License: CC 0
Data sources: Datacite
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ZENODO
Article . 2015
License: CC 0
Data sources: ZENODO
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Identifying Novel P53-Target Genes And Their Functional Analysis

descriptionPublicationkeyboard_double_arrow_right Article 15 May 2015Publisher:Zenodo
Authors: sprotocols;

doi: 10.5281/zenodo.17654

Identifying Novel P53-Target Genes And Their Functional Analysis

Abstract

This protocol describes a procedure to search for novel p53 target genes. First, screen for potential p53 target gene using p53MH algorithm. Then, test these candidate genes using a series of molecular biological assays, including Chromatin immunoprecipitation assay, luciferase reporter activity assay, and regulation of the expression levels of mRNA and protein of these genes by p53 protein. This protocol also includes some assays to measure the mouse implantation and reproduction used in our study.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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