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Direct Hapten Coated Elisa For Immunosensing Of Low Molecular Weight Analytes

descriptionPublicationkeyboard_double_arrow_right Article 15 May 2015Publisher:Zenodo
Authors: Sprotocols;

doi: 10.5281/zenodo.17653

Direct Hapten Coated Elisa For Immunosensing Of Low Molecular Weight Analytes

Abstract

We report a protocol that employs direct coating of smaller hapten on microtiter ELISA plates for the detection of low molecular weight analytes such as pesticides in an immunoassay format. In this method, the polystyrene surface of microtiter plates was functionalize with amino groups using 3-aminoprpyltriethoxysilane (APTES) for the covalent linkage to small molecular hapten with carboxyl groups. The developed immunoassay format could be used as convenient quantitative tool for the sensitive and rapid screening of pesticides and other low molecular weight analytes in samples.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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