This study assessed knowledge of prostate cancer and uptake of screening of prostate cancer among men in abuloma, Port Harcourt L.G.A. of Rivers State, Nigeria. The primary objectives were to evaluate the level of knowledge about prostate cancer, determine screening uptake prevalence, and investigate perceptions and attitudes toward screening. The study also explored the relationship between knowledge and screening uptake, identified barriers and facilitators to screening, and examined the influence of demographic factors. A cross-sectional research design was employed, with a sample size of 382 adult males aged 40 and above involved in lower cadre jobs. Data were collected via questionnaires and analyzed using descriptive statistics and chi-square tests, with logistic regression applied for multivariate analysis at a 0.05 alpha level. The findings revealed low levels of prostate cancer knowledge, with 95.5% of respondents demonstrating poor understanding. Screening uptake was extremely low, with only 0.56% of men having undergone prostate cancer screening. Men with higher knowledge were significantly more likely to undergo screening (χ² = 45.863, p < .001), while socio-demographic factors such as age, marital status, household size, income, and years of residence did not significantly influence screening uptake. Educational level and occupation were the key predictors of screening behavior. The study highlighted the critical need for community health outreach to raise awareness about prostate cancer screening and reduce barriers to participation.