1. Title of Dataset: Raw images dataset associated with "High-speed whole-brain imaging in Drosophila" Gauthey et al. Nat Comm. 2025 The dataset contains 300 tiff files acquired with a custom 2Photon microscope run by the acquisition software ScanImage (MBF). Each file contains 1568 frames: 28 time points of 28 z-planes (from deep to shallow) in 2 channels (green and red). The order is zct (z-planes, channels, time points). Acquisition was conducted at 28Hz volume rate. 1 tiff file contains ~1 second of data. Metadata, including all microscope acquisition parameters, can be accessed through Matlab and ScanImage routines. 2. Date of data collection (range) : 2024-03-01 to 2025-03-01 Links to publications that cite or use the data: https://www.biorxiv.org/content/10.1101/2025.06.18.660371 METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: All details of the data acquired are included in this publication demonstrating Light Bead Microscopy applied to Drosophila brain- "High-speed whole-brain imaging in Drosophila" Gauthey et al. https://www.biorxiv.org/content/10.1101/2025.06.18.660371v1 2. Methods for processing the data: Fully described in "High-speed whole-brain imaging in Drosophila" Gauthey et al. https://www.biorxiv.org/content/10.1101/2025.06.18.660371 3. Instrument- or software-specific information needed to interpret the data: Fully described in "High-speed whole-brain imaging in Drosophila" Gauthey et al. https://www.biorxiv.org/content/10.1101/2025.06.18.660371 4. Standards and calibration information, if appropriate: Fully described in "High-speed whole-brain imaging in Drosophila" Gauthey et al. https://www.biorxiv.org/content/10.1101/2025.06.18.660371 5. Environmental/experimental conditions: Fully described in "High-speed whole-brain imaging in Drosophila" Gauthey et al. https://www.biorxiv.org/content/10.1101/2025.06.18.660371 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: Wayan Gauthey, Stephan Thiberge, Albert Lin