The paper presents data on the results of studying the effect of nisin on the growth and survival of female and male Australian red-claw crayfish Cherax quadricarinatus. The experiment was conducted in closed water supply systems on individuals with clearly expressed signs of sexual dimorphism and divided into control and experimental groups. Nisin, a representative of the class of lantibiotics type A, was added to the diet of the experimental groups in an amount of 0.1 %. The duration of the experiment was 30 days. As a result, an improvement in growth indicators was recorded: the average weight of females in the experimental group increased by 33.6 %, males – by 26.1 % compared to the control group. Survival also increased to 86.7 % for females and 83.3 % for males against 56.7 % and 76.7 %, respectively, in the control groups. The data obtained are of practical value for the development of environmentally friendly technologies in industrial crustacean breeding.