Mumiyo (Shilajit) is a natural herbo-mineral compound long used in traditional medicine for its rejuvenating and adaptogenic effects. This study aimed to evaluate the diuretic activity of Khamzaabad mumiyo in dogs following intravenous administration. Experiments were conducted under controlled conditions, and urine samples were collected hourly for five hours. Mumiyo was administered intravenously at doses of 1–50 mg/kg in 0.9% saline solution. The results showed a clear, dose-dependent increase in urine output and electrolyte excretion, beginning one hour after injection and lasting up to four hours. The diuretic action was primarily associated with reduced tubular reabsorption and enhanced renal excretion of water, sodium, and potassium. These findings suggest that Khamzaabad mumiyo exhibits significant diuretic and natriuretic properties and may be useful for identifying and standardizing Central Asian mumiyo preparations.