This factsheet presents DUC5: Ecosystem-based spatial planning and MPA management, one of the Demonstrator Use Cases (DUCs) developed within the DTO-BioFlow project. It is intended for marine spatial planners, MPA (Marine Protected Area) managers, environmental agencies and researchers engaged in ecosystem-based marine planning and the management of protected areas. DUC5 seeks to enhance ecosystem-based management by developing a species traits database that links benthic species traits to ecosystem services, enabling more effective spatial planning and management of Marine Protected Areas (MPAs). The Demonstrator Use Cases (DUCs) are at the core of DTO-BioFlow, showcasing how biodiversity observations, AI models, and the Digital Twin of the Ocean (DTO) can deliver digital solutions for marine sustainability.