The renovation rate of the building stock must increase to reach the 2050 climate goals. One of the methods to reach this goal, is to shift the focus to district renovations. Therefore the Horizon Europe Mission Project Neutralpath has been initiated. This project investigates how PCEDs (Positive and Clean Energy Districts) can be achieved in 5 European cities, in an inclusive manner. PCEDs are districts that produce more energy than they consume, and only use 'clean', i.e. renewable energy. The design of these PCEDs is done through participative and human-centre principles. This research is part of the Neutralpath project and investigates the most optimal renovation scenarios in existing districts in a holistic manner, with the Muide Meulestede district in Ghent as case study. Energy use, the environmental impact over the full life cycle and costs will be considered. As sub-research, the impact of integrating dynamic simulations in LCA is investigated, with a focus on the impact of occupant behaviour. The study finds that both comfort preference levels and insulation strategies (choice of insulation + finishing materials) significantly affect the total environmental impact of renovation scenarios, and thus the most optimal renovation scenario. Further, comparing dynamic simulation results with those of the Belgian LCA tool TOTEM reveals large discrepancies due to simplified energy use calculations used in TOTEM.