Annotation sets for six ant species, Doleromyrma darwiniana, Iridomyrmex anceps, Linepithema humile, Liometopum microcephalum, Ochetellus glaber, and Tapinoma melanocephalum. Annotations were generated using Braker v.3.0.8.The assembly used for D. darwiniana is generated in this study, whereas the remaining assemblies were downloaded from GenBank. GenBank accessions for the assemblies used in the remaining annotation sets are: I. anceps - GCA_048542785.1, L. humile - GCA_040581485.1, L. microcephalum - GCA_048542565.1, O. glaber - GCA_048543655.1, and T. melanocephalum - GCA_048542345.1. The annotations are provided in the GTF files attached, in addition to the OrthoFinder (version 3.0.1b) results describing individual orthogroup sequences in FASTA format (compressed in Dolichoderinae_orthogroups.tar.gz) and overall copy number counts of all species for all orthogroups (Orthogroups.GeneCount.tsv). The assembly for D. darwiniana is submitted to GenBank, however, its processing is delayed potentially until the end of the governmental shutdown in the United States of America. For this reason, the D. darwiniana genome assembly used in this dataset is provided here in the interim (Doleromyrma_darwiniana.fa).