The report povides a comprehensive overview of the 2025 edition of the xTEF event, held in Milan on 1–2 October 2025. Bringing together policymakers, industry leaders, researchers, and innovators, the event showcased the progress and impact of the four European AI Testing and Experimentation Facilities (TEFs) — agrifoodTEF, TEF-Health, AI-MATTERS, and CitCom.AI. The report highlights key discussions, success stories, and outcomes from each session, demonstrating how TEFs are driving the adoption of trustworthy, human-centric AI across strategic sectors. It also reflects on future directions for strengthening collaboration within Europe’s AI ecosystem, including synergies with CoordinaTEF, the coordination project supporting all four TEFs.