The effluent discharge of synthetic dyes, notably azo dyes such as the anionic dye Reactive Red 195 (RR195), in textile effluents constitutes a significant environmental and public health risk. This is attributed to their inherent toxicity, environmental persistence, and potential carcinogenicity. The assessment of hemp seed oil cake (HSOC), a sustainable and inexpensive agricultural byproduct, as a novel effective adsorbent for the sequestration of the reactive azo dye RR195 from aqueous media was investigated. The influence of critical operational parameters on adsorption performance was systematically examined through a series of batch experiments. The investigation established the following optimal conditions for maximum sequestration: an initial dye concentration of 40 mg/L, an adsorbent dosage of 1.5 g, a temperature of 50°C, a solution pH of 4, and a contact time of 120 minutes under agitation at 100 rpm. The experimental data demonstrated that unmodified HSOC is a highly efficient, renewable, and cost-effective adsorbent, representing its potential as a promising green technology for advanced textile effluent remediation.