This document presents the standard operating protocol (SOP) developed in WP1 for suspect and non-target screening (NTS) analysis of runoff samples using liquid chromatography-high resolution mass spectrometry (LC-HRMS). It is the result of further developments of the preliminary SOP presented in D1.1. The final SOP has been tested for analysis of runoff samples collected in WP1 and implemented for developing an inventory of contaminants of emerging concern (CECs) (T1.3) in urban runoff as well as to assess the fate of pollutants (T1.4). Furthermore, it will be used to analyze around 100 samples from across Europe in relation to the PARC project where UCPH is participating.